SUMMARY, EXPLANATION AND LIMITATIONS:
WT 1 is a gene involved in the induction of Wilms Tumor. The WT 1 gene, located on 11p13, is inactivated in 5 to 10% of sporadic Wilms tumors and in nearly 100% of Denys-Drash patients. In normal tissues, WT 1 (mRNA) has been observed in human kidney, spleen and gonadal ridge mesoderm. The WT 1 gene has also been observed in sertoli cells of testes and in granulosa cells of the ovary. In tumors, WT 1 has been demonstrated in Wilms tumors and in the majority or mesotheliomas (nuclear and paranuclear staining). WT 1 has also been demonstrated in the majority of acute leukemias, but not in cells from chronic myelogenous leukemia. Cytoplasmic staining has been observed in some cases of adenocarcinoma and may represent cross-reactivity with an epitope unrelated to WT 1.
Immunogen: Truncated human WT 1 protein corresponding to aa 1-181.
Staining pattern: Nuclear.
Positive control: Tissue sample from Wilms tumor or mesothelioma.
This antibody is designed for the specific localization of human WT 1 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.