SUMMARY, EXPLANATION AND LIMITATIONS:
Synaptophysin is a synaptic vesicle glycoprotein weighing 38 kDa. It is present in endocrine cells, the brain, spinal cord, and adrenal glands. It acts as a marker for neuroendocrine cells.
Synaptophysin reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary, thyroid, lung, pancreas and gastrointestinal mucosa. Positive staining is seen in neurons of the brain, spinal cord, retina, and Paneth’s cells in the gastrointestinal tract and gastric parietal cells. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely-granular cytoplasmic staining is observed and probably correlates with the distribution of the antigen within neurosecretory vesicles. The expression of Synaptophysin is independent of the presence of NSE or other neuroendocrine markers. Synaptophysin is an independent broad-range marker of neural and neuroendocrine differentiation.
Immunogen: A synthetic peptide from C-terminus of human synaptophysin.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from pancreas or neuroendocrine tumors.
This antibody is designed for the specific localization of human synaptophysin using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
It is a very useful marker for identifying normal and neoplastic neuroendocrine cells. This antibody reacts with neuroendocrine cells of the adrenal medulla, carotid bodies, skin, pituitary gland, thyroid, lung, pancreas and gastrointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastoma, ganglioneuromas, pheochromocytomas.