SUMMARY, EXPLANATION AND LIMITATIONS:
D2-40 reacts with an O-linked sialoglycoprotein (MW 40K) found on lymphatic endothelium, fetal testis and on the surface of testicular germ-cell tumors. D2-40 is also a new selective marker of lymphatic endothelium in normal tissues and vascular lesions. In recent studies, clone D2-40 has shown staining reaction in lymphatic channel endothelium, but not in the adjacent capillary. In the same study, Clone D2-40 has shown staining in endothelium of lymphangiomas whereas hemangiomas, glomus tumors, angiolipomas, pyogenic granulomas and vascular malformations did not show any staining. D2-40 has also been shown to react with Kaposi’s sarcoma and a subset of angiosarcomas. Proliferation and distribution of lymphatic vessels may also be related to prognosis and early metastasis. D2-40 has also been shown to be a very specific marker for malignant mesothelioma.
Immunogen: Oncofetal antigen M2A.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human podoplanin using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The expression in lymphatic endothelium and its absence in venous and arterial vascular endothelium is helpful for the differential diagnosis of vascular tumors. D2-40 is positive for Dabska lymphangiomas tumor (Endovascular papillary angioendotelioma) in 50% of angiosarcomas and over 95% of Kaposi sarcomas and shows no reactivity with hemangiomas, glomus tumor and hemangioendothelioma kaposiform.
It recognizes testicular germ cell tumors but not adult gonadal tissue. In normal central nervous system is positive in choroid plexus, ependymal cells and leptomeninges subependimarias areas. In normal cerebellar cortex stained Purkinje cells and dendritic projections. Hemangioblastomas are positive for D2-40. It has been shown that the identification of lymphatic vascular invasion identified by this monoclonal gastric adenocarcinomas with stage (N0) has negative prognostic value.