SUMMARY, EXPLANATION AND LIMITATIONS:
MiTF is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer MiTF form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.
Immunogen: N-terminal fragment of human MiTF protein.
Staining pattern: Nuclear.
Positive control: Tissue sample from skin or melanoma.
This antibody is designed for the specific localization of human MiTF using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.