SUMMARY, EXPLANATION AND LIMITATIONS:
HMB45 reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium (RPE), and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas.
Immunogen: Extract of pigmented melanoma metastases from lymph nodes.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from melanoma.
This antibody is designed for the specific localization of neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody can be useful in identifying melanomas and other tumors with melanocyte differentiation. In general, tumors of epithelial, lymphoid, glial and mesenchymal are negative.