SUMMARY, EXPLANATION AND LIMITATIONS:
Langerin (CD 207) is a 40 kDa protein, a group II transmembrane oligosaccharide receptor expressed by epidermal Langerhans cells. This protein has an unique carbohydrates-binding site (CRD), which with recognize mannose residues. Langerin has a transmembrane segment, an extracellular and intracellular region. It is directly related with Birbecks granules. It is also involved in the superficial cellular material uptake of the immature dendritic cells. Dendritic cells are antigen presenting cells (APC) which are needed to initiate a T cell-mediated specific immune response. Dendritic cells are localized in no-lymphoid tissues; they are immature cells, whose primary function is the antigen uptaking through the special endocytic structures in their specialized surface membrane or through macropinocytosis. Dentritic cells migrate to secondary lymphoid tissues maturing until they become an efficient antigen presenting cell. During the mature process, dendritic cells loose adhesion receptors, E-Cadherins like molecules and Birbeck’s granules disappear. Although langerin is surface localized, it can be quickly uptaken. In fact, langerin is a powerful membrane superposition inductor and zipping, forming Birbeck’s granules. It has been suggested that these granule induction is a consequence of the antigen capturating function from langerin, allowing a no-classic antigen processing.
Immunogen: Recombinant protein of 29kDa corresponding to the external domain of human Langerin.
Staining pattern: Cell membrane and granule cytoplasmic.
Positive control: Tissue sample from skin.
This antibody is designed for the specific localization of human langerin using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.