SUMMARY, EXPLANATION AND LIMITATIONS:
Recognizes a protein of 67kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F.
This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Immunogen: Synthetic peptide derived from C-terminal of human estrogen receptor.
Staining pattern: Nuclear.
Positive control: Tissue sample breast carcinoma.
This antibody is designed for the specific localization of human ER using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The antibody identify ER in normal or neoplastic cells and reacts positively with secretory epithelial cells of the mammary gland and endometrial lining of the uterus, as well as benign and malignant tumors dependents.