SUMMARY, EXPLANATION AND LIMITATIONS:
Epithelial Membrane Antigen (EMA) antibody is a mucin-like glycoprotein, shown to be useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and squamous epithelium. Hepatocellular Carcinoma, Adrenal Carcinoma and Embryonal Carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms. The absence of EMA can also be of value since negative EMA is characteristic of some tumors including Adrenal Carcinoma, Seminomas, Paraganglioma and Hepatoma.
Isotype: IgG2a kappa.
Immunogen: Delipidated extract of human milk fat globule membranes.
Staining pattern: Cell membrane and cytoplasm.
Positive control: Tissue sample from breast or colon.
This antibody is designed for the specific localization of human EMA using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.