SUMMARY, EXPLANATION AND LIMITATIONS:
E-cadherin is a glycoprotein of the cellular surface with a molecular weight of 123 kDa. It is also known as CD324, Cadherin-1, Uvomorulin, L-CAM, cell-CAM 120/80, and Arc-1. Like other cadherins, E-cadherin is responsible for the calcium dependent adhesion between epidermal cells and other epithelial cell types. The loss of cell adhesion caused by E-cadherin is closely connected to the progression of various carcinomas. This for example was shown in breast or bladder carcinomas. About 45% of the tumours of various organs show reduced expression of E-cadherin. E-cadherin expression in carcinomas has an inversely proportional relationship to the extent of differentiation. In breast carcinoma a correlation was observed between low expression of E-cadherin, lymph node metastases and poor prognosis. Most lobular carcinomas display a complete loss of E-cadherin expression. The immunohistochemical detection of E-cadherin leads to a strong staining of the cytoplasmic membrane. However, an abnormal expression has also been observed inside the cytoplasm of tumour cells, which can suggest a negative prognosis (Nakopoulou et al.).
Isotype: Rabbit IgG
Immunogen: Recombinant protein according to external región of E-cadherin.
Staining pattern: Cell membrane.
Positive control: Tissue sample from tonsil, breast cancer.
This antibody is designed for the specific localization of human E-Cadherin using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.