SUMMARY, EXPLANATION AND LIMITATIONS:
Cytokeratin 34βE12 is a High Molecular Weight cytokeratin that reacts with all squamous and ductal epithelium and stains carcinomas. This antibody recognizes cytokeratins 1, 5, 10, and 14 that are found in complex epithe-lia. Cytokeratin 34βE12 shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules or endometrial glands; there has been no reactivity with cells derived from simple epithelia. Nerve cells, glial cells and mesenchymal tissue such as blood vessels containing only non-keratin types of intermediate filaments are not labelled; however, reactivity with smooth-muscle cells has been occasionally observed. Mesenchymal Tumors, Lymphomas, Melanomas, Neural Tumors and Neuroendocrine Tumors are unreactive with this antibody. Cytokeratin 34βE12 has been shown to be useful in distinguishing Prostatic Adenocarcinoma from Hyperplasia of the Prostate.
Immunogen: Solubilized keratin extracted from human stratum corneum.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from prostate.
This antibody is designed for the specific localization of human cytokeratin HMW using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody is used to identify breast carcinomas and adenocarcinomas of the pancreas, bile ducts, salivary gland carcinomas and transitional epithelium.