SUMMARY, EXPLANATION AND LIMITATIONS:
Cytokeratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Cytokeratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
Immunogen: Synthetic peptide from the C-terminus of human cytokeratin 16.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from squamous cell carcinoma.
This antibody is designed for the specific localization of human cytokeratin 16 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.