SUMMARY, EXPLANATION AND LIMITATIONS:
CDX2 is a transcription factor which regulates proliferation and differentiation of intestinal mucosa. In adults the expression of CDX2 is usually limited to intestinal epithelium. Immunohistochemically, the protein is detectable in nuclei of normal intestinal epithelium. Loss of CDX2 correlates with loss of differentiation in colorectal tumours. Anti-CDX2 antibody is very useful in identifying the origin of metastatic adenocarcinomas and carcinoids. Some mucinous carcinomas of the ovary also stain positive with this CDX2 antibody as well as some carcinomas from the upper gastroinstestinal tract (see Moskaluk et al. 2003).
Immunogen: A synthetic peptide corresponding to residues near the N-terminal of human CDX2.
Staining pattern: Nuclear.
Positive control: Tissue sample from colon or colon carcinoma.
This antibody is designed for the specific localization of human CDX2 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The detection of this antigen has proven useful in identifying the origin of adenocarcinomas and metastatic carcinoid. Some mucinous carcinomas of the ovary also stained positively with this antibody, and also, some carcinomas of the upper gastrointestinal tract.