SUMMARY, EXPLANATION AND LIMITATIONS:
CD7 is a member of the superfamily of genes of immunoglobulins. Its ligand is the molecule K-12 (CD7L) and their activation is critical in signal transduction in T and NK cells, expression of adhesion molecules and regulation of secretion of interferons α and γ. This antibody recognizes a 40kDa glycoprotein of molecular weight identified as CD7 (also known as TP40 and Leu9), positioned at 17q25.2-q25.3 and present on thymocytes, mature T cells and NK cells. During T cell differentiation, one of the earliest markers of lineage appear, so that constitutes the most clinically useful marker for acute lymphoid leukemia origin T. By contrast, the CD7 molecule is absent in some cases of severe combined immunodeficiency.
Immunogen: A synthetic peptide derived from an internal region of Human CD7 protein.
Staining pattern: Cell membrane.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human CD7 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody is useful for the identification of lymphoid neoplasms derived from them as the CD7 antigen is expressed in mature and immature T cells and NK cells, even with mixed myeloid immunophenotype (precursor NK cell leukemia / myeloid). However, it should be noted that in the peripheral T cell lymphomas frequently arise deletion or posttranscriptional regulation of pre-and CD7 with loss of expression in the cell membrane so their absence does not exclude the source of T neoplasia. Also, it is exceptional in benign inflammatory dermatosis reactive T cells do not express CD7 so these histopathologic entities should be considered in the differential diagnosis.