SUMMARY, EXPLANATION AND LIMITATIONS:
CD68 is a transmembrane glycoprotein of 110kD, with a large expression in monocytes and human tissues. Is a member of the family of lysosomal membrane-associated glycoprotein / endosomal (LAMP). The protein located mainly lysosomes and endosomes with a circulating fraction to the cell surface. Is an integral membrane protein with a Type I strongly glycosylated extracellular domain which binds to lectins or tissues and specific organs selectins. The protein is also a member of the family of scavenger receptors. The typical function of scavenger receptors is to clean the cell debris, and promotion of phagocytosis mediate the recruitment and activation of macrophages. There results of alternative connections in multiple transcripts that encode different isoforms.
Immunogen: Subcellular fraction of human alveolar macrophages.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from tonsil or lymph node.
This antibody is designed for the specific localization of human CD68 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia.