SUMMARY, EXPLANATION AND LIMITATIONS:
CD45 is a complex molecule and is comprised of different glycoproteins ranging from 180-240 kDa. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms (e.g., naive T-cells and memory T-cells). CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
CD45RA antibody reacts with mature, non-activated T and B-cells. CD45RA is also reactive with medullary thymocytes, mantle-zone lymphocytes in follicles of lymph nodes, spleen and lymphocytes of the paracortex. CD45RA shows no reactivity with cortical thymocytes, immature T-cells or activated B-cells in germinal centers.
Immunogen: Stimulated human leukocyte.
Staining pattern: Cell membrane.
Positive control: Tissue sample from tonsil or lymph node.
This antibody is designed for the specific localization of human CD45RA using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The anti-CD45RA antibody is useful in the study of lymphocyte subpopulations in autoimmune diseases, especially inflammatory bowel disease, and immunophenotypic characterization of leukemias and lymphomas.