SUMMARY, EXPLANATION AND LIMITATIONS:
The CD43 antigen (synonyms: leukosialin, gp95, sialophorin, leucocyte sialoglycoprotein) is an integral typ-1 membrane protein with molecular weight of about 95-110 kDa. It belongs to the family of cell surface mucins and functions in T-cell immunity and cell-cell interactions. CD43 is expressed on most T-cells, on activated B-cells, NK-cells, granulocytes, macrophages, and megakaryocytes. Resting B-cells are CD43 negative.
Immunogen: Pluripotent human cell line K562.
Staining pattern: Cell membrane.
Positive control: Tissue sample from tonsil.
This antibody is designed for the specific localization of human CD43 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
In immunohistochemistry CD43 is used to identify and classify T-cell neoplasias. 80 – 90 % of all T-cell lymphomas, mantel cell lymphomas, Burkitt lymphomas and B-cell SLL are CD43 positive. ALL and the majority of AML are also positive too.