SUMMARY, EXPLANATION AND LIMITATIONS:
CD31 is a glycoprotein expressed on endothelial cells and in platelets. It is known to be involved in cell signaling and cell adhesion. CD31 reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells.1It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils. Antibody to CD31 is of value in the study of benign and malignant vascular tumors. Staining for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Immunogen: Membrane preparation of a spleen from a patient with hairy cell leukemia.
Staining pattern: Cell membrane.
Positive control: Tissue sample from placenta.
This antibody is designed for the specific localization of human CD31 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The anti-CD31 antibody is useful for identify benign and malignant tumors of vascular origin. Other tumors and pseudotumoral lesions are also frequently positive against CD31. CD31 staining has also been used to assess angiogenesis in tumors as predictor of recurrence.