SUMMARY, EXPLANATION AND LIMITATIONS:
The antibody reacts with a formalin-resistant epitop of the CD20-antigen which is found on most B-cells. CD20 is therefore called pan-B-cell marker. The antibody detects a polypeptide of 33 kDa and a smaller cellular antigen of 30 kDa. The antibody derived from clone L26 is the best and most specific pan-B-cell antibody. It shows no cross-reactivity with non-lymphatic tissue.
Isotype: I IgG2a/kappa
Immunogen: B-cells from human tonsil.
Staining pattern: Predominantly membranous with occasional intracytoplasmic extension including stain of perinuclear region and the Golgi apparatus.
Positive control: Tissue sample from tonsil, lymph node or human spleen.
This antibody is designed for the specific localization of human CD20 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
The antibody is useful for identifying lymphoid malignancies of B origin, whether non-Hodgkin lymphomas, acute or chronic lymphocytic leukemia B and hairy cell leukemia. Also, anti-CD20 antibody also stained tumor cells of Hodgkin’s disease nodular lymphocyte-predominant subtype paragranuloma and occasionally, the Reed-Sternberg cells of other histological types of Hodgkin’s disease.