SUMMARY, EXPLANATION AND LIMITATIONS:
CD10 (CALLA, Common Acute Lymphoblastic Leukemia Antigen) is an integral Type II-membrane protein with a molecular weight of 100 kDa. CD10 was identified as a human membrane-associated neutral metalloendopeptidase.
Synonyms are NEP, encephalokinase or neprilysin.
CD10 is present on precursor B-cells, a subset of precursor T-cells, mature granulocytes, approximately 75 % of B-ALL, a subsetof T-ALL/T-LBL, and on all ALL-subtypes. Burkitt’s lymphomas and myelomas stain positive for CD10, as well as some diffuse large-cell B-cell lymphomas and most follicular lymphomas. Few tumours of epithelial origin like carcinomas of the kidney, bladder, prostate, uterus and liver stain also positive.
MALT lymphomas and mantel cell lymphomas are negative for CD10.
CD10 is a frequently utilised marker for differential diagnosis of lymphomas but is also used for discrimination of e.g. hepatocellular carcinomas vs. liver metastases of other origin.
Immunogen: Recombinant protein according to the external domain of human CD10.
Staining pattern: Cell membrane.
Positive control: Tissue sample from small intestine or kidney.
This antibody is designed for the specific localization of human CD10 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
CD10 was identified initially as a common acute lymphoblastic leukemia specific antigen (CALLA). However, subsequent studies have shown that CD10 is expressed on the surface of a wide variety of normal and neoplastic cells. In malignant lymphomas, this antigen is expressed in lymphoblastic lymphomas, germinal center, Burkitt’s lymphoma and in patients with chronic myeloid leukemia (CML). In addition, CD10 has been identified in the lymphoid progenitor cell surface, immature B cells in bone marrow of adult and germinal center B cells of lymphoid tissue. Also expressed in non-lymphoid cells and tissues such as breast myoepithelial cells, bile canaliculi, fibroblasts, and particularly high expression in the brush border of renal tubular cells and intestinal epithelial cells.