SUMMARY, EXPLANATION AND LIMITATIONS:
Caldesmon is a developmentally regulated protein involved in smooth muscle and nonmuscle contraction. Two closely related variants of human caldesmon have been identified which differ in their eletrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in nonmuscle tissue and cells. Neither of the two variants has been detected in skeletal muscle.
Immunogen: Crude human uterus extract.
Staining pattern: Cytoplasmic.
Positive control: Tissue sample from uterus.
This antibody is designed for the specific localization of human caldesmon HMW using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
This antibody may be useful for the study of muscle contraction and is a useful marker of neoplasms of smooth muscle. Is not expressed on cells or tumors myofibroblast derived therefrom. The cases of nodular fasciitis, fibromatosis, and myofibroblastic sarcomas are negative for h-caldesmon.