SUMMARY, EXPLANATION AND LIMITATIONS:
Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB. Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptormediated lymphocyte proliferation and signaling to NF-B, which controls the expression of genes critical for cell survival, proliferation, and immune responses. Defects in Bcl-10 have been identified in various types of cancer.
Immunogen: Recombinat protein of human Bcl-10.
Staining pattern: Cytoplasmatic and nuclear.
Positive control: Tissue sample from tonsil, MALT lymphoma.
This antibody is designed for the specific localization of human Bcl-10 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
In normal lymphoid tissue, anti-Bcl-10 reacts with germinal center B cells, marginal zone and sporadically and weakly with B lymphocytes of the mantle. In normal cytoplasmic expression is detected in lymphoid organs (spleen, tonsil, lymph node and thymus), gut lymphoid tissue (appendix) and luminal border of epithelial cells in the breast.
The nuclear expression of Bcl-10 is useful in the diagnosis of MALT lymphoma by showing the translocation t (1; 14) (p22, q32). Unlike MALT lymphomas, B-cell lymphomas of follicular center lymphoma and nodal large B-cell expressed Bcl-10 in the nucleus only at 20 and 10% of cases respectively. Mantle B lymphomas do not express bcl-10 in the nucleus.